Affinity chromatography

Biochemical characterization of recombinant serotonin N-acetyltransferase

Engineering / Biochemistry / Thermodynamics / Technology / Catalysis / Kinetics / Virology / Calcium / Biological Chemistry / HIV / Aspergillus / Biological Sciences / Saccharomyces cerevisiae / PCR / Ubiquitin / Plant Genome Project / Tuberculosis / Humans / Sequence alignment / Mycobacterium tuberculosis / Escherichia coli / Mice / CPM / Female / Animals / Male / Glutathione / Physical sciences / Candida albicans / Phosphorylation / Proteins / Capsid / Affinity chromatography / SALT TOLERANCE / Enzyme / Casein Kinase 2 / Cattle / Molecular cloning / Rats / HeLa cells / Substrate Specificity / Pineal / Protein Conformation / Protein Kinase / Amino Acid Sequence / Base Sequence / Ribonucleoproteins / Amino Acid Substitution Rates / Recombinant Protein / Recombinant Proteins / Biological Macromolecules / Hydrogen-Ion Concentration / Asparagine / Fusion Protein / DNA binding proteins / Site-directed Mutagenesis / Sodium Chloride / Biochemistry and cell biology / Dimerization / Okadaic acid / Molecular Sequence Data / DNA mutational analysis / Kinetics / Virology / Calcium / Biological Chemistry / HIV / Aspergillus / Biological Sciences / Saccharomyces cerevisiae / PCR / Ubiquitin / Plant Genome Project / Tuberculosis / Humans / Sequence alignment / Mycobacterium tuberculosis / Escherichia coli / Mice / CPM / Female / Animals / Male / Glutathione / Physical sciences / Candida albicans / Phosphorylation / Proteins / Capsid / Affinity chromatography / SALT TOLERANCE / Enzyme / Casein Kinase 2 / Cattle / Molecular cloning / Rats / HeLa cells / Substrate Specificity / Pineal / Protein Conformation / Protein Kinase / Amino Acid Sequence / Base Sequence / Ribonucleoproteins / Amino Acid Substitution Rates / Recombinant Protein / Recombinant Proteins / Biological Macromolecules / Hydrogen-Ion Concentration / Asparagine / Fusion Protein / DNA binding proteins / Site-directed Mutagenesis / Sodium Chloride / Biochemistry and cell biology / Dimerization / Okadaic acid / Molecular Sequence Data / DNA mutational analysis

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COMMON ACUTE LYMPHOCYTIC LEUKEMIA ANTIGEN IS IDENTICAL TO NEUTRAL ENDOPEPTIDASE

Humans / The / Affinity chromatography / Molecular cloning / Experimental Medicine / Amino Acid Sequence / Base Sequence / Acute Lymphocytic Leukemia / DNA probes / Molecular Sequence Data / Amino Acid Sequence / Base Sequence / Acute Lymphocytic Leukemia / DNA probes / Molecular Sequence Data

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A proteomic approach to identify phosphoproteins encoded by cDNA libraries

Analytical Chemistry / Proteomics / Polymerase Chain Reaction / Phage display / Iron / Affinity chromatography / Large Scale / Analytical Biochemistry / Fusion Protein / cDNA library / Biochemistry and cell biology / Tyrosine Phosphorylation / Affinity chromatography / Large Scale / Analytical Biochemistry / Fusion Protein / cDNA library / Biochemistry and cell biology / Tyrosine Phosphorylation

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Identification of amino acid residues critical for endonuclease and integration activities of HIV-1 IN protein in Vitro

Bacteriology / Catalysis / Flow Cytometry / Virology / Calcium / Biological Chemistry / Magnetic Resonance Spectroscopy / Gene expression / Cell separation / Biocatalysis / Signal Transduction / HIV / Biological Sciences / Cell line / Identification / Humans / Mutagenesis / Sequence alignment / Mutation / Placenta / Virus / Escherichia coli / Animals / Plants / Monoclonal Antibodies / Chemotaxis / Phosphorylation / Plasmids / Affinity chromatography / Phenotype / Phosphotyrosine / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Mycoplasma Genitalium / Lysine / Amino Acid Profile / Transfection / Protein Conformation / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Chemokines / Structure activity Relationship / Newcastle Disease Virus / Recombinant Proteins / Protein Binding / Hydrolysis / DNA binding proteins / Site-directed Mutagenesis / Interleukin / Interferon Alpha / Down-Regulation / Molecular Sequence Data / Biological Chemistry / Magnetic Resonance Spectroscopy / Gene expression / Cell separation / Biocatalysis / Signal Transduction / HIV / Biological Sciences / Cell line / Identification / Humans / Mutagenesis / Sequence alignment / Mutation / Placenta / Virus / Escherichia coli / Animals / Plants / Monoclonal Antibodies / Chemotaxis / Phosphorylation / Plasmids / Affinity chromatography / Phenotype / Phosphotyrosine / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Mycoplasma Genitalium / Lysine / Amino Acid Profile / Transfection / Protein Conformation / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Chemokines / Structure activity Relationship / Newcastle Disease Virus / Recombinant Proteins / Protein Binding / Hydrolysis / DNA binding proteins / Site-directed Mutagenesis / Interleukin / Interferon Alpha / Down-Regulation / Molecular Sequence Data

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Identification of amino acid residues critical for endonuclease and integration activities of HIV1 IN protein in Vitro

Bacteriology / Catalysis / Flow Cytometry / Virology / Calcium / Biological Chemistry / Magnetic Resonance Spectroscopy / Gene expression / Cell separation / Biocatalysis / Signal Transduction / HIV / Biological Sciences / Cell line / Identification / Humans / Mutagenesis / Sequence alignment / Mutation / Placenta / Virus / Escherichia coli / Animals / Biological / Plants / Monoclonal Antibodies / Chemotaxis / Phosphorylation / Plasmids / Affinity chromatography / Phenotype / Phosphotyrosine / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Mycoplasma Genitalium / Lysine / Amino Acid Profile / Transfection / Protein Conformation / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Chemokines / Structure activity Relationship / Newcastle Disease Virus / Recombinant Proteins / Protein Binding / Hydrolysis / DNA binding proteins / Site-directed Mutagenesis / Interleukin / Interferon Alpha / Down-Regulation / Molecular Sequence Data / Biological Chemistry / Magnetic Resonance Spectroscopy / Gene expression / Cell separation / Biocatalysis / Signal Transduction / HIV / Biological Sciences / Cell line / Identification / Humans / Mutagenesis / Sequence alignment / Mutation / Placenta / Virus / Escherichia coli / Animals / Biological / Plants / Monoclonal Antibodies / Chemotaxis / Phosphorylation / Plasmids / Affinity chromatography / Phenotype / Phosphotyrosine / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Mycoplasma Genitalium / Lysine / Amino Acid Profile / Transfection / Protein Conformation / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Chemokines / Structure activity Relationship / Newcastle Disease Virus / Recombinant Proteins / Protein Binding / Hydrolysis / DNA binding proteins / Site-directed Mutagenesis / Interleukin / Interferon Alpha / Down-Regulation / Molecular Sequence Data

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Heterologous expression in Escherichia coli of Neurospora crassa neutral trehalase as an active enzyme

Gene expression / Multidisciplinary / Neurospora crassa / Biological Sciences / Vancomycin / Aquaporins (AQP) / Mutagenesis / Copper / Escherichia coli / Animals / Polymerase Chain Reaction / Temperature / Phosphorylation / Biochemical / Affinity chromatography / Enzyme / CHEMICAL SCIENCES / Cattle / Molecular cloning / Protein expression and purification / Fluorescent Antibody Technique / Anti-Bacterial Agents / Active site / Rats / Specific Activity / Time Factors / Filamentous fungi / Protein Expression / Metal ion / Amino Acid Sequence / Base Sequence / Recombinant Protein / Heterologous Expression / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Glycosyl Hydrolases / Biochemistry and cell biology / Molecular Sequence Data / Aquaporins (AQP) / Mutagenesis / Copper / Escherichia coli / Animals / Polymerase Chain Reaction / Temperature / Phosphorylation / Biochemical / Affinity chromatography / Enzyme / CHEMICAL SCIENCES / Cattle / Molecular cloning / Protein expression and purification / Fluorescent Antibody Technique / Anti-Bacterial Agents / Active site / Rats / Specific Activity / Time Factors / Filamentous fungi / Protein Expression / Metal ion / Amino Acid Sequence / Base Sequence / Recombinant Protein / Heterologous Expression / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Glycosyl Hydrolases / Biochemistry and cell biology / Molecular Sequence Data

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Cloning, expression, purification, and characterization of Vibrio cholerae transcriptional activator, HlyU

Technology / High Pressure / Biotechnology / Protein / Glycobiology / Cytotoxicity / Biological Sciences / Escherichia coli / Bacteria / Physical sciences / Molecular Biotechnology / Expression / Affinity chromatography / Ion Exchange Chromatography / Enzyme / Protein expression and purification / Enzyme activity / Amino Acid Profile / Molecular Mass / Molecular weight / Protein Kinase / Amino Acid Sequence / Open Reading Frame / Reaction Mechanism / Recombinant Protein / Polyacrylamide Gel Electrophoresis / Fusion Protein / Full Length Movies / Biochemistry and cell biology / DNA sequence / Cytotoxicity / Biological Sciences / Escherichia coli / Bacteria / Physical sciences / Molecular Biotechnology / Expression / Affinity chromatography / Ion Exchange Chromatography / Enzyme / Protein expression and purification / Enzyme activity / Amino Acid Profile / Molecular Mass / Molecular weight / Protein Kinase / Amino Acid Sequence / Open Reading Frame / Reaction Mechanism / Recombinant Protein / Polyacrylamide Gel Electrophoresis / Fusion Protein / Full Length Movies / Biochemistry and cell biology / DNA sequence

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Cloning, Expression, Purification, and Characterization of a Novel Esterase from Lactobacillus plantarum

Technology / High Pressure / Biotechnology / Protein / Glycobiology / Cytotoxicity / Biological Sciences / Escherichia coli / Bacteria / Physical sciences / Molecular Biotechnology / Expression / Affinity chromatography / Ion Exchange Chromatography / Enzyme / Protein expression and purification / Enzyme activity / Amino Acid Profile / Molecular Mass / Molecular weight / Protein Kinase / Amino Acid Sequence / Open Reading Frame / Reaction Mechanism / Recombinant Protein / Polyacrylamide Gel Electrophoresis / Fusion Protein / Full Length Movies / Biochemistry and cell biology / DNA sequence / Cytotoxicity / Biological Sciences / Escherichia coli / Bacteria / Physical sciences / Molecular Biotechnology / Expression / Affinity chromatography / Ion Exchange Chromatography / Enzyme / Protein expression and purification / Enzyme activity / Amino Acid Profile / Molecular Mass / Molecular weight / Protein Kinase / Amino Acid Sequence / Open Reading Frame / Reaction Mechanism / Recombinant Protein / Polyacrylamide Gel Electrophoresis / Fusion Protein / Full Length Movies / Biochemistry and cell biology / DNA sequence

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Inhibitory activity of 6-O-angeloylprenolin fromCentipeda minima on farnesyl protein transferase

Enzyme Inhibitors / Magnetic Resonance Spectroscopy / Methanol / Asteraceae / Affinity chromatography / Sesquiterpenes / High Pressure Liquid Chromatography / Plant extracts / Quntitative Thin Layer Chromatography / Lactones / Solvents / Sesquiterpenes / High Pressure Liquid Chromatography / Plant extracts / Quntitative Thin Layer Chromatography / Lactones / Solvents

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An antibody-based affinity chromatography tool to assess Cu, Zn superoxide dismutase (SOD) G93A structural complexity in vivo

Biotechnology / Proteomics / Industrial Biotechnology / Environmental Biotechnology / Western blotting / Brain / Humans / Liver / Medical Biotechnology / Animals / Affinity chromatography / Superoxide Dismutase / Rats / Structural Complexity / Protein Conformation / Two-Dimensional Gel Electrophoresis / Recombinant Proteins / Brain / Humans / Liver / Medical Biotechnology / Animals / Affinity chromatography / Superoxide Dismutase / Rats / Structural Complexity / Protein Conformation / Two-Dimensional Gel Electrophoresis / Recombinant Proteins

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Expression of β-galactosidase and β-xylosidase genes during microspore and pollen development

Plant Biology / Tobacco / Arabidopsis thaliana / P-glycoprotein / POLLEN GERMINATION / Affinity chromatography / Pollen analysis / Enzyme / Cell Wall / Planta / Cell Expansion / Affinity chromatography / Pollen analysis / Enzyme / Cell Wall / Planta / Cell Expansion

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Tristetraprolin recruits functional mRNA decay complexes to ARE sequences

Western blotting / Cellular / Humans / Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis / Affinity chromatography / HeLa cells / Tumor necrosis factor-alpha / Tristetraprolin / Transfection / Cell free System / Protein Binding / Biochemistry and cell biology / Interleukin / HeLa cells / Tumor necrosis factor-alpha / Tristetraprolin / Transfection / Cell free System / Protein Binding / Biochemistry and cell biology / Interleukin

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Acetylcholinesterases from Elapidae snake venoms: biochemical, immunological and enzymatic characterization

Catalysis / Enzyme Inhibitors / Biological Sciences / Physical sciences / Acetylcholinesterase / Monoclonal Antibodies / Affinity chromatography / Enzyme / Snake Venom / Active site / Protein Conformation / Monoclonal Antibody / Bovine Serum Albumin / Monoclonal Antibodies / Affinity chromatography / Enzyme / Snake Venom / Active site / Protein Conformation / Monoclonal Antibody / Bovine Serum Albumin

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Transcription Factor IIIBGenerates Extended DNA Interactions in RNA Polymerase IIITranscription Complexes on tRNAGenes

Cellular Biology / RNA polymerase / Transcription Factors / Biological Sciences / Saccharomyces cerevisiae / Molecular and cellular biology / Plasmids / Affinity chromatography / Transcription Factor / Triazines / Base Sequence / Protein Binding / Molecular and cellular biology / Plasmids / Affinity chromatography / Transcription Factor / Triazines / Base Sequence / Protein Binding

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Subcellular localization of an extracellular serine protease in Leishmania (Leishmania) amazonensis

Microbiology / Parasitology / Medical Microbiology / Animals / Host-parasite interactions / Affinity chromatography / Enzyme / Subcellular Localization / Veterinary Sciences / Lysosomes / Enzymatic Activity / Serine Protease / Immunoblotting / Affinity chromatography / Enzyme / Subcellular Localization / Veterinary Sciences / Lysosomes / Enzymatic Activity / Serine Protease / Immunoblotting

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Production of Recombinant Human DNA Polymerase Delta in a Bombyx mori Bioreactor

Biotechnology / DNA replication / DNA repair / Gene expression / Multidisciplinary / Western blotting / Humans / Animals / Affinity chromatography / PLoS one / Enzyme / HeLa cells / Larva / Bioreactors / Hemolymph / Genetic Recombination / Molecular weight / Large Scale / Subcellular Fractions / Recombinant Proteins / Base Excision Repair / DNA Polymerase / Bombyx mori / Western blotting / Humans / Animals / Affinity chromatography / PLoS one / Enzyme / HeLa cells / Larva / Bioreactors / Hemolymph / Genetic Recombination / Molecular weight / Large Scale / Subcellular Fractions / Recombinant Proteins / Base Excision Repair / DNA Polymerase / Bombyx mori

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